Comparision of ABO system and Rh(D) antigen between the manual microplate and automatic microplate
Abstract
Comparision was made of the results of manual microplate and automatic microplate determination for ABO system and Rh(D) antigen in 127 specimens blood of donated. A 1% cell suspension in 0.85% normal saline solution was used in the manual microplate and a 1.5% cell suspension in ethylenediamine tetraacetate/normal saline solution (EDTA/NSS) was used in the automatic microplate. Both techniques used anti-A 1 : 64, anti-B 1 : 32, anti-A, B 1 : 32 and anti-D 1 : 64. Identical results were obtained for each sample tested by both techniques, A Rh(D) positive 28 specimens, B Rh(D) positive 35 specimens, O Rh(D) positive 49 specimens, AB Rh(D) positive 13 specimens, B Rh(D) negative 1 specimen and O Rh weak D Ag 1 specimen. The modified microplate technique is simple, accurate and economical. The automatic microplate is suitable for a mass blood grouping and also reduces the risk of infection.
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