The evacuated blood collection system is a method of venipuncture used worldwide nowadays. Since all medical equipment should be tested for effectiveness and safety, the test for contamination in vacuum blood collection is necessary. Here, we report the results from a study of contamination in the vacuum tube. A short evaluation of bacterial contamination in 100 vacuum tubes was performed, with null prevalence of bacterial contamination observed. This could mean that the used vacuum tubes were sterile, or contaminated but not detected due to small sample in this study.