The purpose of this research was to study the effect of refrigerated cold pack compression in minimizing pain from cloxacillin intravenous injection of pediatric patients and factors related to pain. The subjects were 40 children, 6-15 years of age, admitted to the pediatric wards 1 and 2 at Songklanagarind Hospital, and receiving cloxacillin intravenous injection every 6 hours. Each patient was and was not compressed with refrigerated cold pack during cloxacillin intravenous injection every 6 hours. After each injection, the researchers recorded the level of pain immediately. Pomnirun' Pain Measuring Scale (PPMS) was used to assess pain. The results revealed that pediatric patients who received refrigerated cold pack compression while receiving cloxacillin injection reported statistically significant lower pain levels than when they were not compressed at p < .001 and there was no significant difference in the level of pain between age, sex, drug experiences, or amount of drug whether being compressed with cold pack or not.