The counting of leukocytes and erythrocytes in cerebrospinal fluid (CSF) is still performed microscopically, e.g., using a chamber, in most laboratories. This requires sufficient practical experience, is time-consuming, and may constitute a problem in emergency diagnostics. Recently, following advances in flow cytometry technology, some reports have mentioned the use of flow cytometry analyzer as a good alternative for CSF cell count. In this article, we report our experience with the flow cytometry pattern of cryptococcus meningitis in 4 HIV-infected patients. In general, the parameters showed a lymphocytic profile with basophilia. The flow cytometry pattern presented in this study is the first report. Although there are some limitations due to the nature of a preliminary report, we decided to report these data to encourage other research groups to study this topic.