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Home > Vol 32, No 4 (2014) > Sinkhan

The 2012 Interlaboratory Comparison of Forensic DNA Typing in Thailand

Nonglak Sinkhan, Weawgarn Duangshatome, Wiparat Jaitrong, Kwanjai Kumyos, Rapeeporn Khienthong, Chalampoo Wongvoravivat, Tanin Bhoopat, Kanha Muisuk, Janpen Thanakitgosate, Chairat Manasatienkij, Kawin Rasmeepaisarn, Jintana Pradutkanchana, Suwit Rueangkittisakul, Budsaba Rerkamnuaychoke, Sukone Pradutkanchana

Abstract

Objective: To evaluate the results of the 2012 interlaboratory comparison for forensic deoxyribonucleic acid (DNA) typing including autosomal short tandem repeat loci (aSTR), Y-chromosome short tandem repeat loci (Y-STR), X-chromosome short tandem repeat loci (X-STR) and mitochondrial DNA (mtDNA).

Material and Method: The analysis was performed using 4 blood samples stored on FTA® cards. Twelve laboratories in Thailand participated in this study. The results from each participant were compared with the consensus results.

Results: The analysis of aSTR, Y-STR, X-STR and mtDNA were performed in 12 (100.0%), 8 (66.7%), 6 (50.0%) and 7 (58.3%) laboratories, respectively. All DNA profiles of aSTR, Y-STR and X-STR from all the participating laboratories showed the same STR profiles. However, the calculation of forensic DNA typing statistical values and the format of laboratory reports among these laboratories showed several variations. Only the mtDNA test showed some variations among the laboratories.

Conclusion: For aSTR, Y-STR and X-STR tests, the trainings in statistical calculation of forensic DNA typing are required. For mtDNA test, workshop training should be established. Furthermore, a standard guideline for forensic DNA typing reports should be released in order to provide all the necessary information in the laboratory reports.

 Keywords

การเปรียบเทียบระหว่างห้องปฏิบัติการ; นิติเวชศาสตร์; ประเทศไทย; รูปแบบดีเอ็นเอ; DNA typing; forensic; interlaboratory comparison; Thailand

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About The Authors

Nonglak Sinkhan
Sub Division of Forensic Biochemistry, Institute of Forensic Medicine, Police General Hospital, Royal Thai Police, Pathum Wan, Bangkok, 10330, Thailand.
Thailand

Weawgarn Duangshatome
Biology and DNA Sub-Division, Central Scientific Crime Detection Division, Office of Forensic Science, Royal Thai Police, Pathum Wan, Bangkok, 10330, Thailand.
Thailand

Wiparat Jaitrong
DNA and Biological Identification Unit, Forensic Science Police Center 3, Muang, Nakhonratchasima, 30000, Thailand.
Thailand

Kwanjai Kumyos
DNA and Biological Identification Unit, Forensic Science Police Center 5, Muang, Lampang, 52000, Thailand.
Thailand

Rapeeporn Khienthong
DNA and Biological Identification Unit, Forensic Science Police Center 10, Hat Yai, Songkhla, 90110, Thailand.
Thailand

Chalampoo Wongvoravivat
Bureau of Forensic Biology, Central Institute of Forensic Science, Ministry of Justice, Lak Si, Bangkok, 10210, Thailand.
Thailand

Tanin Bhoopat
Forensic Genetics Unit, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Muang, Chiang Mai, 50200, Thailand.
Thailand

Kanha Muisuk
Department of Forensic Medicine, Faculty of Medicine, Khon Kaen University, Muang, Khon Kaen, 40002, Thailand.
Thailand

Janpen Thanakitgosate
Human Genetics Unit, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Ratchathewi, Bangkok, 10400, Thailand.
Thailand

Chairat Manasatienkij
Division of Forensic Serology, Department of Forensic Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok Noi, Bangkok, 10700, Thailand.
Thailand

Kawin Rasmeepaisarn
Serology Unit, Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Pathum Wan, Bangkok, 10330, Thailand.
Thailand

Jintana Pradutkanchana
Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Thailand

Suwit Rueangkittisakul
Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Thailand

Budsaba Rerkamnuaychoke
Human Genetics Unit, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Ratchathewi, Bangkok, 10400, Thailand.
Thailand

Sukone Pradutkanchana
Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Thailand

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