Glucometer, a point-of-care testing device for determination of blood glucose level, is widelyused in medical clinic and for self-monitoring blood glucose. Accuracy of the measurement results depend on user skill and the analytical performance of the glucometer. Analytical interferences of glucometer is one of the most easily overlooked problem among users, although it has been reported that hematocrit, ascorbic acid, acetaminophen (paracetamol) and icodextrin interfere inblood glucose determination. This article aims to describe the effect of these interfering substances on glucometer. This information may be useful to prevent the false interpretation hypoglycemic or hyperglycemic status in suspected patients.