PSU CalPat v 1.4 has been developed several new functions for statistical value estimations in forensic deoxyribonucleic acid (DNA) typing. These include the editable prior probability value and expanded statistical value calculations from the usage of autosomal short tandem repeat, such as 1) parentage testing in trios of alleged father, mother and child 2) avuncular testing in trios of alleged uncle, mother and child 3) paternal incest testing or maternal incest testing in trios of father, mother and child 4) sibling incest testing in trios of father, mother and child 5) incest testing in duos of mother and child 6) first cousin testing. This software can print out a DNA typing report of various cases and can show the interpretation in Thai language. All statistical values are estimated by using allele frequencies from Thai population. Thus, PSU CalPat v 1.4 is one of the software of choice for Thai forensic DNA scientists.